Microplastic particles induce endothelial activation

Background and Aims : Although microplastics being omnipresent in our environment already detected various organisms, less is known about the effects of on vascular biology particular. Here we investigated carboxylated polystyrene (PS) microplastic particles (1 ?m) murine endothelial immune cells, which are both crucially involved inflammation.Methods: Murine monocytic J774A.1 cells MyEND were stimulated with PS (3/6 hrs, n=7-10). mRNA protein levels analyzed using real-time PCR ELISA. Adhesion assays performed incubating confluent Calcein-AM labelled (static/flow). Adherent by fluorescence microscopy (n=6). C57BL/6J mice injected TRITC-conjugated i.v. (n=6/group). localized blood liver IHC staining.Results: In induced Il1-? Tnf-? expression TNF-? release. up-regulated Icam-1 Vcam-1 sVCAM-1 adhesion monolayers under static as well flow conditions. vivo, taken up neutrophiles (Ly6G-positive) peripheral blood. accumulated liver, accompanied a strong up-regulation acute phase proteins Saa1, Saa2 Saa3. Moreover, Aortic tissue from showed enhanced Il-1? expression.Conclusions: able to activate endothelium subsequent monocyte adhesion, representing hallmark inflammation. Thus, need be evaluated potential novel environmental risk factor for Methods: staining. Results: expression. Conclusions: